Coding

Part:BBa_K4963000:Design

Designed by: Tanish Gupta, Vansh Jain, Rohit Chebbi   Group: iGEM23_BITSPilani-Goa-India   (2023-10-11)


Cannabinoid Receptor 1 (Modified)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 345
    Illegal PstI site found at 1044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 345
    Illegal PstI site found at 1044
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 345
    Illegal PstI site found at 1044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 345
    Illegal PstI site found at 1044
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 843


Design Notes

The domain to be shed was identified as the separate floating lobe opposite the free end (as against the binding end) of the primary group. Care was taken to ensure that none of the active binding sites were disturbed, nor the integrity of the protein compromised. The entire cylindrical structure was allowed to ensure that fusion with other parts is possible.


Source

Cannabinoid receptor 1 (CB1) is a part of the Endocannabinoid system, a signaling mechanism in the Central nervous system.1 It was determined to be the ideal receptor to the drug we were using in our project - honokiol, which is a STAT3 inhibitor. The receptor possesses binding sites for both honokiol and AM - 6538 (antagonist); notably in the same region.

References

1. Busquets Garcia A, Soria-Gomez E, Bellocchio L, Marsicano G. Cannabinoid receptor type-1: breaking the dogmas. F1000Res. 2016 

  May 24;5:F1000 Faculty Rev-990. doi: 10.12688/f1000research.8245.1. PMID: 27239293; PMCID: PMC4879932.